Can someone answer the question and provide a short explanation? Thanks! Which of the following statements…

Can someone answer the question and provide a short explanation?
Thanks!
Which of the following statements is NOT correct regarding
protein seperation techniques?
A) Size-exclusion chromatography works on the same principle as
polyacrylamide gel electrophoresis
B) Specific antibodies are required for immunoblotting, antibody
affinity chromatography as well as immunoprecipitation
C) Two-dimensional gel electrophoresis is conceptually the same
as combining gel filtration chromatography and ion exchange
chromatography
D) Western blotting is a useful technique to isolate proteins
for use in downstream assays